S. Girardo

Cell viscosity influences hematogenous dissemination and metastatic extravasation of tumor cells

This study shows that tumor cell viscosity affects their ability to circulate and extravasate, influencing metastasis.

Endothelial calcium firing mediates extravasation of metastatic tumor cells.

This study identifies endothelial calcium signaling as a key driver of tumor cell extravasation through mechanistic and imaging analyses in zebrafish embryos.

High-throughput viscoelastic characterization of cells in hyperbolic microchannels

This paper introduces hyperbolic microchannels for high-throughput viscoelastic measurement of cells and soft particles, showing accurate recovery of mechanical properties under controlled stress.

Viscoelastic properties of suspended cells measured with shear flow deformation cytometry

This paper introduces a microfluidic method to measure viscoelastic properties of suspended cells using shear flow deformation cytometry and demonstrates cell cycle-dependent rheological behavior.

Correlative all-optical quantification of mass density and mechanics of sub-cellular compartments with fluorescence specificity

This paper introduces FOB microscopy to measure sub-cellular density and mechanics with fluorescence specificity, revealing differences between nucleoplasm and cytoplasm.

Using real-time fluorescence and deformability cytometry and deep learning to transfer molecular specificity to label-free sorting

3D microenvironment stiffness regulates tumor spheroid growth and mechanics via p21 and ROCK

This study shows that 3D microenvironment stiffness affects tumor spheroid growth and mechanics through p21 and ROCK pathways.

Polyacrylamide Bead Sensors for in vivo Quantification of Cell-Scale Stress in Zebrafish Development

The paper develops PAAm bead sensors to measure cell-scale stress in zebrafish embryos and uses COMPAX to detect stress patterns during development.

Standardized microgel beads as elastic cell mechanical probes

This paper presents standardized poly-acylamide microgel beads as mechanical probes for cell mechanics, showing they can be calibrated to mimic cell elasticity and used for cross-comparison of measurement techniques.

Real-time fluorescence and deformability cytometry - flow cytometry goes mechanics

This paper introduces RT-FDC, combining real-time fluorescence imaging with deformability cytometry to enable label-free, high-throughput mechanical and molecular cell analysis.