S. Shekhar

Twinfilin is a non-processive depolymerase which synergizes with formin to dramatically accelerate actin filament uncapping by 300-fold

Multicomponent depolymerization of actin filament pointed ends by cofilin and cyclase-associated protein depends upon filament age

This study shows cofilin and CAP promote depolymerization of ADP-Pi actin filaments, though at lower rates than ADP filaments, suggesting new pathways for actin filament disassembly.

Functional redundancy and formin-independent localization of tropomyosin isoforms in Saccharomyces cerevisiae

This study shows that Tpm isoforms in yeast bind actin filaments independently of formin isoforms and exhibit functional redundancy in actin cable maintenance.

Cyclase-associated protein is a pro-formin anti-capping processive depolymerase of actin barbed and pointed ends

This study reveals that CAP is a processive depolymerase of actin filaments, removing subunits from both barbed and pointed ends, and interacts with formin and capping protein.

HAMLET, a human milk protein-lipid complex, modulates amoxicillin-induced changes in an ex vivo biofilm model of the oral microbiome

This study shows that HAMLET combined with amoxicillin reduces oral biofilm viability and increases Lactobacillus crispatus, suggesting synergistic antimicrobial effects in a polymicrobial model.

Trem2 Agonist Reprograms Foamy Macrophages to Promote Atherosclerotic Plaque Stability

This study shows that a Trem2 agonist increases plaque macrophage survival and proliferation while improving plaque stability by enhancing collagen deposition and reducing necrotic core size.

Multicomponent regulation of actin barbed end assembly by twinfilin, formin and capping protein

This study reveals how twinfilin, formin, and capping protein interact at actin barbed ends to regulate polymerization, showing twinfilin promotes formin-based elongation when CP is present.

Cooperative hydrodynamics accompany multicellular-like colonial organization in the unicellular ciliate Stentor

This study shows that hydrodynamic coupling in Stentor colonies enhances feeding efficiency, with asymmetric benefits depending on individual solitary feeding rates.

Single-molecule imaging of IQGAP1 regulating actin filament dynamics in real time

This study uses single-molecule TIRF microscopy to show IQGAP1 regulates actin filament dynamics by dimerizing, binding filament sides, and transiently capping barbed ends, with distinct domains controlling each activity.

Mouse IgG2a antibodies specific for the commensal Streptococcus mitis show stronger cross-reactivity with Streptococcus pneumoniae than IgG1 antibodies

Direct visualization of how Actin Depolymerizing Factor’s filament severing and depolymerization synergizes with Capping Protein's "monomer funneling" to promote rapid polarized growth of actin filaments

This study directly visualizes how ADF's filament severing and depolymerization synergize with Capping Protein's monomer funneling to enhance actin filament growth rates.