Y.-W. Chang

Centromeric chromatin clearings demarcate the site of kinetochore formation

The paper uses cryo-ET to reveal the architecture of centromeric chromatin clearings and their role in kinetochore formation and amplification.

Native ultrastructure of fresh human brain vitrified directly from autopsy revealed by cryo-electron tomography with cryo-plasma focused ion beam milling

This paper presents a method to image unfixed, never frozen human brain tissue using cryo-ET with xenon plasma FIB milling, revealing native ultrastructures like myelin and autophagy components.

Inflammasomes primarily restrict cytosolic Salmonella replication within human macrophages

This study identifies caspase-1 and GSDMD as key mediators of inflammasome responses that restrict Salmonella replication in human macrophages.

Engineered retrovirus-like nanocarriers for messenger RNA delivery into neurons

This paper develops engineered retrovirus-like nanocarriers for efficient mRNA delivery into neurons by modifying extracellular vesicles with Arc protein capsids and RNA elements.

Dynamic structural adaptations enable the endobiotic predation of bdellovibrio bacteriovorus

This study uses cryo-electron tomography to image the predation cycle of Bdellovibrio bacteriovorus, revealing structural adaptations during prey invasion and replication.

An apical membrane complex controls rhoptry exocytosis and invasion in Toxoplasma

The paper identifies CRMPs as a complex involved in rhoptry exocytosis and invasion in Toxoplasma, acting as a host-molecular sensor.

Bacterial outer-membrane polysaccharide export (OPX) proteins occupy three structural classes with selective β-barrel porin requirements for polymer secretion

The paper identifies three structural classes of bacterial OPX proteins and shows that some use β-barrel porins for polysaccharide secretion when they cannot span the outer membrane.

Omicron-specific mRNA vaccine induced potent neutralizing antibody against Omicron but not other SARS-CoV-2 variants

This study evaluates Omicron-specific mRNA vaccines and finds they induce strong neutralizing antibodies against Omicron but not other variants, while bivalent vaccines show broader neutralizing activity.

In situ structure and priming mechanism of the rhoptry secretion system in Plasmodium revealed by cryo-electron tomography

This study uses cryo-electron tomography to reveal the structure and priming mechanism of the rhoptry secretion system in Plasmodium falciparum merozoites, identifying distinct organizational states and conformational changes in the RSA.

Nanoscale details of mitochondrial fission revealed by cryo-electron tomography

This study uses cryo-electron tomography to reveal nanoscale details of mitochondrial fission in mammalian cells, showing coordinated membrane constriction and the role of actin and septin filaments.

A novel widespread bacterial structure related to the flagellar type III secretion system

The paper identifies a novel widespread bacterial structure related to the flagellar type III secretion system, suggesting its involvement in diverse cellular functions.

Bacterial flagellar motor PL-ring disassembly sub-complexes are widespread and ancient

The paper shows that bacterial flagellar motor PL-ring sub-complexes persist after disassembly across multiple phyla and are ancient, not recently transferred.

Simulations suggest a constrictive force is required for Gram-negative bacterial cell division

The paper uses simulations to show that a constrictive force is needed for Gram-negative bacterial cell division, as cell wall remodeling alone is insufficient.

In vivo structures of the Helicobacter pylori cag type IV secretion system

This study uses electron cryotomography to reveal the in vivo structure of the Helicobacter pylori cag T4SS, showing its architecture and proposed delivery mechanism.

In vivo structures of an intact type VI secretion system revealed by electron cryotomography

This study uses electron cryotomography to visualize the extended type VI secretion system in intact bacterial cells, revealing new structural features and insights into its function.

Uncharacterized bacterial structures revealed by electron cryotomography

The paper presents uncharacterized bacterial structures observed via electron cryotomography to encourage their identification and study.

Structure of the archaellar motor and associated cytoplasmic cone in Thermococcus kodakaraensis

This study uses electron cryotomography to visualize the archaellar motor and cytoplasmic cone in Thermococcus kodakaraensis, revealing structural differences from bacterial type IV pilus.

Structure of the Legionella Dot/Icm type IV secretion system in situ by electron cryotomography

The paper reports the in situ structure of the Legionella Dot/Icm T4BSS using electron cryotomography, revealing structural similarities to T4ASSs and highlighting type-specific features.