A. Villa

Molecular dynamics study of stiffness and rupture of axonal membranes

This study uses molecular dynamics to investigate how lipid composition and protein concentration affect axonal membrane rupture, finding that node-of-Ranvier membranes rupture at lower strains than myelin.

Imaging minimal bacteria at the nanoscale: a reliable and versatile process to perform Single Molecule Localization Microscopy in mycoplasmas

This paper develops a reliable protocol for Single Molecule Localization Microscopy in mycoplasmas, enabling high-resolution imaging of subcellular structures.

Antibody-based delivery of Interleukin-9 to neovascular structures: therapeutic evaluation in cancer and arthritis

This study evaluates the therapeutic potential of IL9 delivered via antibody-based fusion proteins in cancer and arthritis models, finding modest anti-tumor activity but no benefit in arthritis.

Sortase-mediated site-specific modification of interleukin-2 for the generation of a tumor targeting acetazolamide-cytokine conjugate

This paper develops a tumor-targeting cytokine conjugate using Sortase A-mediated site-specific modification of interleukin-2 with acetazolamide, targeting carbonic anhydrase IX.

Immunotherapy with immunocytokines and PD-1 blockade enhances the anticancer activity of small molecule-drug conjugates targeting carbonic anhydrase IX

This study shows that combining SMDCs targeting carbonic anhydrase IX with immunotherapy enhances anticancer activity in mouse models of renal cell carcinoma and colorectal cancer.

The immunocytokine L19-TNF eradicates Sarcomas in combination with Chemotherapy or with immune check-point inhibitors

The paper shows that combining the immunocytokine L19-TNF with chemotherapy or immune checkpoint inhibitors improves tumor remission in a sarcoma mouse model.

Localized axolemma deformations suggest mechanoporation as axonal injury trigger

This study uses simulations to show that axonal injury can result from membrane pore formation due to localized deformations, aligning with known injury thresholds.

A novel fully-human potency-matched dual cytokine-antibody fusion protein targets carbonic anhydrase IX in renal cell carcinomas

This paper describes the development and characterization of a dual cytokine-antibody fusion protein targeting carbonic anhydrase IX in renal cell carcinomas, showing tumor localization and growth inhibition.

Targeted enhancement of the therapeutic window of L19-TNF by transient and selective inhibition of RIPK1-signaling cascade

This study shows that transient RIPK1 inhibition increases the tolerated dose of L19-TNF while preserving its antitumor effects and tumor targeting.

The antibody-based delivery of interleukin-12 to solid tumors boosts NK and CD8+ T cell activity and synergizes with immune check-point inhibitors

This paper describes a fusion protein that delivers interleukin-12 to tumors, enhancing NK and CD8+ T cell activity and synergizing with checkpoint inhibitors.

Molecular basis of egg coat cross-linking sheds light on ZP1-associated female infertility

The paper identifies ZP1 cross-linking as crucial for human ZP assembly and explores its modulation by fucosylation and zinc sparks, suggesting it as a target for non-hormonal contraception.