Y. Yoshikuni

Label-free structural imaging of plant roots and microbes using third-harmonic generation microscopy

This study uses third-harmonic generation microscopy to image plant roots and microbes in their natural environment without labels.

Multi-substrate specificity shaped the complex evolution of the aminotransferase family across the tree of life

The paper traces the evolutionary history of aminotransferases across species, revealing their substrate promiscuity and how it shaped nitrogen metabolic networks.

Crystal Structure of the 4-Hydroxybutyryl-CoA Synthetase (ADP-forming) from Nitrosopumilus maritimus

The paper presents the crystal structure of Nmar_0206 from Nitrosopumilus maritimus, revealing a conserved interdomain linker loop that influences homodimer stability and contributes to the enzyme's energy efficiency in the 3HP/4HB cycle.

Inter-subunit coupling enables fast CO2-fixation by reductive carboxylases

This paper investigates the structural and dynamic mechanisms of CO2 fixation by ECRs, revealing inter-subunit coupling that enables fast catalytic activity.

Structural Insights into Bifunctional Thaumarchaeal Crotonyl-CoA Hydratase and 3-Hydroxypropionyl-CoA Dehydratase from Nitrosopumilus maritimus

This study presents the first high-resolution crystal structure of a bifunctional enzyme involved in the 3HP/4HB cycle in Nitrosopumilus maritimus, revealing its dual catalytic activities.

Structural adaptation of oxygen tolerance in 4-hydroxybutyrl-CoA dehydratase, a key enzyme of archaeal carbon fixation

The paper investigates how 4HBD, a key enzyme in archaeal carbon fixation, adapted structurally to become oxygen-tolerant, revealing evolutionary changes that enabled aerobic metabolism.

A plant host enables the heterologous production and combinatorial study of fungal lignin-degrading enzymes

The paper demonstrates that Nicotiana benthamiana can produce fungal lignin-degrading enzymes and shows that peroxidase specialization affects C-C bond cleavage during lignin oxidation.