This study compares FCS and FRAP techniques to measure diffusion in yeast plasma membrane proteins, revealing discrepancies in Sso2 diffusion due to subdomain localization.

bioRxiv · Jul 21, 2026 · PreprintCell Biology

Adapting the OpenFlexure Microscope for Affordable Live-Cell Imaging

Jodie R. Malcolm, Olympia Physouni, Lola K Kelly, Juliana B. T. Carnielli, Sandy J. Macdonald, Mark Bentley, Stephen P Howarth, Stuart Lacy, Alastair Droop, Matthew Reilly, James P J Chong, Ana Paula C A Lima, Jeremy Charles Mottram, L. F. Wiggins, William J. Brackenbury, Peter J O’Toole

A 3D-printed OpenFlexure Microscope adapted for humid incubators, with relocated electronics, ASA printing and 22 Ω current-limiting resistors, records 48-hour label-free docetaxel responses that CellPhe can analyse.

bioRxiv · Jul 17, 2026 · PreprintCell Biology✦ In-depth digest

Ontogeny-independent expression of LPCAT2 in granuloma macrophages during experimental visceral leishmaniasis

Shoumit Dey, Jianhua Cao, Benjamin Balluff, Gaia Mazza, Helen M. Ashwin, Lesley Gilbert, Sally R. James, Adam Dowle, Grant M. Calder, Nidhi Sharma Dey, Peter J O’Toole, Ron M. A. Heeren, Paul Martin Kaye

The paper uses multimodal imaging and single-cell transcriptomics to identify LPCAT2-mediated membrane remodeling in granuloma macrophages during visceral leishmaniasis.

Communications Biology · Mar 20, 2026Peer-reviewedImmunology

Characterising cancer-stroma interactions through high-content phenotyping from microscopy time-lapses

Laura Wiggins, Jodie R. Malcolm, Karen Hogg, Peter J O’Toole, Julie C. Wilson, William J. Brackenbury

This study uses live-cell imaging and automated phenotyping to characterize cancer-stromal interactions, revealing phenotypic changes in breast cancer cells exposed to cancer-associated fibroblasts.

bioRxiv · Oct 2, 2025 · PreprintCancer Biology

CellPhePy: a Python implementation of the CellPhe toolkit for automated cell phenotyping from microscopy time-lapse videos

Laura Wiggins, Stuart Lacy, Graeme Park, Joanne Marrison, Ben Powell, Beth A. Cimini, Peter J O’Toole, Julie C. Wilson, William J. Brackenbury

Journal of Microscopy · Apr 24, 2025Peer-reviewedCell Biology