Peng‐Fei Xia

The paper repurposes marine bacteria to convert lignin-derived carbon into valuable chemicals using seawater, achieving carbon and water conservation.

This study investigates how spatial confinement affects cyanobacteria growth and functionality in engineered living materials, revealing impaired performance and altered physiology.

This paper expands prime editing capabilities by utilizing non-canonical cleavage to enable editing in the 5' direction of a classic nick site.

This paper develops a streamlined prime editing system for Escherichia coli Nissle 1917, achieving high editing efficiencies and reduced off-target effects for DNA deletion, insertion, and substitution.

This paper introduces XSTART, a PAM-independent base editing system for universal gene inactivation in bacteria, achieving near 100% efficiency but with off-target events.

This study reprograms marine bacteria to convert lignin monomers into valuable chemicals with a CRISPRi system, achieving a 4.89-fold increase in protocatechuate yield.

This paper presents a CRISPR-Cas-based system called VADER for degrading antibiotic resistance genes (ARGs) in environmental biotechnology, achieving 100% ARG elimination in a prototype reactor.

This paper develops a base-editing tool for Synechococcus elongatus to enable precise genome modifications, enhancing glycogen content through multiplex editing.

This paper develops a CRISPR-based base-editing tool for acetogenic bacteria to enable precise single-nucleotide modifications without DNA cleavage, improving gene editing efficiency.