Michael A. Laflamme

This paper develops a scalable bioreactor-based method using growth factors to produce human pluripotent stem cell-derived cardiomyocytes with high purity and yield.

This study develops and validates a mathematical model to predict hPSC proliferation in vertical-wheel bioreactors by incorporating shear stress and energy dissipation rate effects, achieving accurate predictions with low error rates.

This paper characterizes the hydrodynamic environment in vertical-wheel bioreactors and shows that trajectory-based energy dissipation rate exposures better predict cell behavior than volume-averaged metrics.

This paper describes a method to generate valvular interstitial cells from human pluripotent stem cells, showing high transcriptional similarity to primary fetal VICs and recapitulating disease features in an in vitro model.

This study generates human pluripotent stem cell-derived AVN-like pacemaker cells that replicate AVN function in vivo, suggesting their potential as biological conduction bridges for treating AV block.

FAST-STEM accelerates human pluripotent stem cell engineering by enabling rapid transgene integration and differentiation into multiple cell types.