Tommaso Patriarchi

This paper introduces a chemigenetic biosensor, WHaloEKAR, that reports ERK activity through fluorescence lifetime changes, enabling spatially resolved and multiplexed measurements in cultured cells and live animals.

The paper introduces PinkyCaMP, a new red fluorescent calcium sensor with improved brightness, photostability, and compatibility with optogenetics and neurotransmitter imaging.

The paper investigates how altered gravity affects adrenergic-mediated cAMP signaling in intact cells, finding shifts in cAMP production EC50 and kinetics under hypergravity and microgravity.

This paper develops an engineered nanobody inhibitor to control opioid receptor function with subcellular precision and demonstrates its effectiveness in vivo.

The paper investigates the spatial and temporal dynamics of noradrenaline release in the primary motor cortex during motor learning, revealing refined spatial patterns and the impact of non-behavior-related activity on learning.

The paper identifies orexin/hypocretin neurons as precise encoders of net body movement across behaviors and metabolic states, independent of head movement and nutritional state.

This study shows that experience-dependent increases in hippocampal sharp-wave ripples are disrupted in 5xFAD Alzheimer's disease mice, suggesting non-cholinergic pathological changes.

The paper investigates how dopamine transmission and mating drive change over days in male mice, finding two mechanisms that regulate these slow-timescale dynamics.

The paper investigates how hypothalamic hypocretin/orexin neurons regulate decision policy complexity through metabolic and neural signaling mechanisms in mice.

This study uses base editing to downregulate PTBP1 in neurons of a PD mouse model, rescuing motor symptoms without affecting astrocytes.

The paper shows that high innervation density of cross-reacting transmitters causes non-specific activation of GPCR-based sensors, requiring specific innervation silencing for accurate interpretation.

This study measures histamine dynamics during feeding in mice and finds rapid fluctuations influenced by solution type and time of day, with regional differences in recovery patterns.

This paper develops a genetically encoded sensor, NOPLight, to detect endogenous N/OFQ release with high spatial and temporal resolution in vitro and in vivo.

This paper quantifies GPCR intrinsic activation at the plasma membrane, revealing spatial multimodality with most receptors showing negligible spontaneous activation.

The paper introduces a novel genetically-encoded sensor for PAC1 receptor activation, demonstrating its utility in vitro and in vivo across species.

This study shows that dopamine release in the lateral hypothalamus (LH) increases orexin neuron activity through dopamine D2 receptors, revealing a new mechanism linking dopamine and reward expectation.

The paper evaluates dLight photometry for measuring dopamine dynamics in the dorsolateral striatum, showing it can detect dopamine increases and transient kinetics during cocaine exposure and conditioning.

This study uses prime editing in mice brains to introduce a mutation in the Adrb1 gene, leading to increased activity and exploratory behavior.

This study shows that tonic and burst firing patterns of the locus coeruleus modulate brain activity and dynamics in mice through distinct mechanisms.

This paper introduces parapinopsin (PPO), a photoswitchable GPCR-based opsin that enables rapid and reversible presynaptic inhibition, and demonstrates its utility in vivo.

The paper shows dopamine modulates PKA in striatal neurons during reward learning, with D1R and D2R neurons responding oppositely to dopamine changes.

This study shows heroin activates specific dopamine neurons in the ventral tegmental area projecting to the medial shell of the nucleus accumbens, and manipulations of these neurons affect heroin reinforcement.