Bernhard Brutscher

Kinetic and Mechanistic Characterization of RSpFAST, A Non-Covalent Versatile Chemogenetic Platform for Fluorescence Imaging

This paper introduces RSpFAST, a non-covalent chemogenetic platform for fluorescence imaging, and demonstrates its dual behavior as a stable label and a reversible photoswitcher.

Structural basis of the two-photon photoactivation mechanism of orange carotenoid protein

The paper elucidates the structural basis of the two-photon photoactivation mechanism of orange carotenoid protein using a combination of structural and biochemical methods.

Decoding mEos4b Day-Long Maturation and Engineering Fast Maturing Variants

The paper characterizes the maturation speed of mEos-derived PCFPs in E. coli and develops fast-maturing variants of mEos4b.

Identification of uranyl-binding proteins in Arabidopsis thaliana cells exposed to uranium: Insights from a metalloproteomic analysis and characterization of Glycine-Rich RNA-binding Protein 7 (GRP7)

This study identifies 57 uranyl-binding proteins in Arabidopsis thaliana cells, with GRP7 showing specific U(VI) binding and interference with RNA binding, suggesting a role in uranium toxicity.

Light-dependent flavin redox and adduct states control the conformation and DNA binding activity of the transcription factor EL222

This study investigates how light-induced changes in the flavin cofactor of EL222 affect its conformation and DNA binding, revealing two distinct lit states and their role in photoactivation.