Francesco Castagnetti

Organotchi automates segmentation and morphological analysis of neural organoids, quantifying features and tracking changes over time to improve quality assessment and downstream analysis.

The paper identifies late endosomes as selective RNA carriers in motor neurons and shows that ALS-linked FUS mutations disrupt this process.

This study compares two single-cell RNA-sequencing multiplexing techniques, 10x and Parse, on cerebellar organoids, finding similar cellular diversity but differences in transcript capture and cell stress.

The paper uses Pepper fluorescent RNA to visualize and study the dynamics of circRNAs and lncRNAs in live cells, revealing their interactions with pathological aggregates and stress granules.

This study examines how ALS-associated FUS mutations alter the RNA and protein composition of Stress Granules in neural cells, revealing molecular changes linked to neurodegeneration.

The paper shows that reducing m6A content via METTL3 knock-down decreases FUS-associated ALS granules in cell models and patient-derived fibroblasts.