Stephen S. Gisselbrecht

This paper characterizes FG214, a heme-binding one-component switch from Fimbriimonas ginsengisoli, showing heme redox state controls monomer-dimer transitions and DNA binding.

The paper identifies non-DNA-contacting residues that determine mono- versus bispecific DNA binding in forkhead transcription factors using protein binding microarrays.

The paper develops PIONEAR-seq to analyze TF binding to nucleosomes and finds that DNA flexibility, not TF intrinsic properties, determines binding preferences.

The paper analyzes DNA binding effects of 92 human homeodomain mutants, identifying 14 novel specificity-determining residues and highlighting limitations of variant effect prediction tools.