The paper develops an MR-based brain template and atlas for optical imaging techniques, enabling anatomical mapping of fluorescent signals in cleared mouse brains.

bioRxiv · Nov 14, 2022 · PreprintNeuroscience

Baculovirus-vectored precision delivery of large DNA cargoes in human genomes

Francesco Aulicino, Martin Pelosse, Christine Toelzer, Julien Capin, Erwin Ilegems, Parisa Meysami, Ruth Rollarson, Per‐Olof Berggren, Mark Simon Dillingham, Christiane Schaffitzel, Moin Ahson Saleem, Gavin Iain Welsh, Imre Berger

This paper develops a baculovirus-based system for delivering large DNA cargoes for CRISPR-based genome editing in human cells, achieving up to 30% correct interventions.

Nucleic Acids Research · Jul 8, 2022Peer-reviewedSynthetic Biology

Transcriptome analysis of EPO and GFP HEK293 Cell-lines Reveal Shifts in Energy and ER Capacity Support Improved Erythropoietin Production in HEK293F Cells

Rasool Saghaleyni, Magdalena Malm, Noah Moruzzi, Jan Zrimec, Ronia Razavi, Num Wistbacka, Hannes Thorell, Anton Pintar, Andreas Hober, Fredrik Edfors, Véronique Chotteau, Per‐Olof Berggren, Luigi Grassi, Aleksej Zelezniak, Thomas Svensson, , Johan Rockberg

The paper compares transcriptomic profiles of HEK293 cell lines producing erythropoietin or GFP to identify factors associated with improved erythropoietin production.

Cell Reports · Jun 1, 2022Peer-reviewedSystems Biology

Islet macrophages drive islet remodeling and compensatory hyperinsulinemia in the early diabetes

Manesh Chittezhath, Divya Gunaseelan, Xiaofeng Zheng, Riasat Hasan, Vanessa S. Y. Tay, Seok Ting Lim, Xiaomeng Wang, Per‐Olof Berggren, Stefan Richard Bornstein, Bernhard Otto Boehm, Christiane Ruedl, Yusuf Ali

The paper shows that islet resident macrophages drive islet remodeling and hyperinsulinemia in early type-2 diabetes by promoting vascularization and insulin secretion.

American Journal of Physiology-Endocrinology and Metabolism · Oct 1, 2019Peer-reviewedMolecular Biology