Mark A. Akeson

This study introduces NRCeq, a method to identify capped full-length SARS-CoV-2 transcripts, revealing new insights into sgRNA structure and expression.

The paper identifies high-confidence full-length human RNA isoform scaffolds using nanopore sequencing by improving 5' cap detection and 3' polyadenylation site identification.

This paper tests the use of Cid1 to add poly(I) tails to RNA for direct nanopore sequencing, enabling detection of both poly(A) and non-poly(A) RNAs and nascent transcripts.

The paper reports a SARS-CoV-2 lineage with mutations from known variants of concern that is rejected by sequence repositories due to a deletion, suggesting underestimation of its frequency.

The paper presents an optimized PromethION nanopore sequencing method and new computational tools for efficient human genome assembly, achieving high accuracy and speed.

The paper uses nanopore sequencing to analyze poly(A) RNA from human cells, identifying long transcripts and modifications not captured by traditional methods.

The paper uses nanopore sequencing to assemble and characterize the centromeric region of a human Y chromosome, overcoming challenges posed by repetitive DNA.

The paper uses long-read RNAseq to identify and quantify complex isoforms in individual B cells, revealing widespread transcriptional variation in surface receptors and the antibody heavy chain locus.