John M. Denu

This study introduces CRISPRm, a dCas9-metabolic enzyme fusion platform, to investigate how nuclear metabolic perturbations affect gene expression and chromatin states in HEK293T cells.

The paper shows methionine, not SAM, is the primary metabolic signal for chromatin remodeling under stress, with methionine depletion causing epigenetic and transcriptional changes independent of SAM levels.

The paper investigates how dietary restriction of specific essential amino acids affects metabolism and chromatin states in mice, finding distinct effects for methionine, leucine, and isoleucine.

This study shows that fasting, not just calorie reduction, is necessary for many benefits of caloric restriction in 3xTg mice with Alzheimer's disease.

The paper identifies that nucleic acid sequences, specifically polyadenine tracks, contribute to the targeting of H2A.Z by the SWR chromatin remodeler beyond canonical NDRs and acetylation sites.

The paper shows that low H3K79 methylation in ESCs promotes transcription elongation at housekeeping genes, and inhibiting DOT1L enhances iPSC reprogramming by increasing H3K9ac.

This study shows that TMAO reduces neurite density and amyloid-β plaque intensity in a mouse model of Alzheimer's disease.

This study shows that SIRT3 deficiency under caloric restriction increases lifespan but decreases aerobic capacity and mitochondrial function.