Kohei Omachi

Anticodon-edited tRNA enables translational readthrough of COL4A5 premature termination codons

The paper demonstrates that ACE-tRNAs enable readthrough of COL4A5 nonsense variants, restoring full-length protein expression and heterotrimer formation in cell models of Alport syndrome.

Basement Membrane Structural Integrity Dictates Trans-Tissue Deposition of Laminin in Mammals

The paper shows that laminin-2 in the kidney glomerular basement membrane comes from circulating sources, not local cells, and that BM integrity affects this trans-tissue deposition.

Epithelium Stratifies via Nucleation and Growth Induced by Foam-Geometric Instability

This study identifies a mechanism for epithelial stratification analogous to a phase transition, driven by foam-geometric instability and nucleation-growth processes.

Defining cellular complexity in human autosomal dominant polycystic kidney disease by multimodal single cell analysis

This study uses single-cell multiomic analysis to define cellular complexity in ADPKD, identifying novel transcriptomic and epigenomic features in cystic kidney cells.

Comparative Analysis and Rational Design of dCas9-VP64 Variants for CRISPR Activation

The paper compares different dCas9-VP64 constructs for CRISPR activation and identifies superior variants that enhance gene activation efficiency.

Aminoglycoside-induced premature termination codon readthrough of COL4A5 nonsense mutations that cause Alport syndrome

This study investigates the feasibility of PTC readthrough therapy for Alport syndrome by testing its effect on COL4A5 nonsense mutations using a luciferase reporter system.