Jeffrey H. Miner

This study identifies three sequential stages of actomyosin remodeling in podocyte injury across multiple kidney diseases, suggesting a structured, potentially reversible response.

The paper shows synaptopodin enables cells to adapt their adhesion structures to resist perpendicular forces by reorganizing the actin cytoskeleton and integrin-based adhesions.

The paper identifies a mechanical homeostasis mechanism in podocytes where balance between fluid shear stress and cellular contractility prevents injury, showing that reducing blood pressure can worsen outcomes when contractility is impaired.

The paper demonstrates that ACE-tRNAs enable readthrough of COL4A5 nonsense variants, restoring full-length protein expression and heterotrimer formation in cell models of Alport syndrome.

This study evaluates genetic testing outcomes for kidney disease, finding 34% of patients had pathogenic variants, 52% had variants of uncertain significance (VUS), and multidisciplinary review with biobank data helped reclassify some VUS.

The paper shows that laminin-2 in the kidney glomerular basement membrane comes from circulating sources, not local cells, and that BM integrity affects this trans-tissue deposition.

This paper describes a U-ExM protocol for analyzing subcellular structures in mouse and human kidney tissues with high detail using conventional microscopy.

This study uses single-cell multiomic analysis to define cellular complexity in ADPKD, identifying novel transcriptomic and epigenomic features in cystic kidney cells.

The paper compares different dCas9-VP64 constructs for CRISPR activation and identifies superior variants that enhance gene activation efficiency.

This study investigates the feasibility of PTC readthrough therapy for Alport syndrome by testing its effect on COL4A5 nonsense mutations using a luciferase reporter system.

The paper identifies BM composition and architecture at inter-tissue interfaces in mouse hair follicles, revealing specialized structures for different interactions.