Mikhail G. Shapiro

This paper introduces a permanent acoustic window for high-resolution, non-invasive ultrasound imaging of human brain activity, demonstrating its use in mapping cortical responses to finger movement.

This paper introduces bicones, tiny acoustic contrast agents based on gas vesicles, showing their effectiveness in imaging and therapy through ultrasound.

This paper introduces a bioorthogonal labeling method to fluorescently visualize gas vesicles in cells, enabling analysis of their size and distribution.

This study demonstrates a closed-loop ultrasonic brain-machine interface that enables nonhuman primates to control movements using real-time neurovascular activity predictions.

This study investigates how gas vesicles interact with blood components and shows that surface modifications can alter these interactions to improve in vivo performance.

This paper introduces a genetically encoded ultrasonic calcium reporter (URoC) for noninvasive in vivo imaging of calcium signaling in mammals.

This study develops a mouse model to study focused ultrasound neuromodulation, showing reduced off-target effects and revealing non-auditory side effects at higher pressures.

The paper introduces SEMPER, a method for expressing multiple proteins from single mRNAs at tunable ratios, demonstrating its use in cell lines and acoustic reporter genes.

This study uses cryo-electron tomography to reveal the structure of Anabaena flos-aquae gas vesicles, showing how GvpA subunits form the shell and how GvpC provides structural reinforcement.

This paper develops a temperature homeostasis mechanism in engineered living materials using E. coli to maintain optimal growth and protein production across seasonal temperature variations.

This study investigates the mechanics of ultrasound neuromodulation in mice, finding that off-target sensory effects depend on frequency and that the vertebral column acts as a waveguide for shear waves.

This paper develops a method to control therapeutic bacteria using focused ultrasound to target tumor sites and release immune checkpoint inhibitors, effectively suppressing tumor growth.

This paper demonstrates the feasibility of using functional ultrasound (fUS) imaging to decode movement intentions for brain-machine interfaces in non-human primates.

This study identifies the mechanism by which focused ultrasound excites neurons through mechanosensitive calcium channels and ion channel amplification.

This paper introduces gas vesicles as ultrasound contrast agents to enhance transcranial hemodynamic functional ultrasound imaging of mouse brain activity.

This paper introduces thermomers, temperature-controlled protein dimerization domains that enable remote control of intracellular interactions in various biological contexts.

This paper demonstrates that genetically encoded gas vesicles can be manipulated by ultrasound to control engineered cells.

This paper demonstrates the use of gas vesicles engineered to produce mechanical effects via ultrasound, enabling targeted cell lysis and therapeutic payload release in vitro, in cellulo, and in vivo.

This paper introduces a noninvasive method to control specific neural circuits using acoustically targeted chemogenetics, demonstrating its ability to modulate hippocampal neurons and affect memory formation in mice.

This study shows ultrasound causes cortical activation through an indirect auditory mechanism, not direct neuromodulation at the ultrasound focus.

This paper introduces a genetically encoded MRI reporter using aquaporin 1 to enhance diffusion weighted MRI contrast without metal ions or toxicity.