Brandon A. Boone

Chromatin-Assisted Targeting Enables Precise DNA Methylation Editing in Plants

SunTag-NOVA, a SunTag-MQ1v editor adding TRBIP1 and an oligomerizing CHLAMY domain, methylates and silences FWA, FT and TMM in Arabidopsis without the genome-wide off-target methylation seen with TRBIP1 alone.

An antagonistic epigenetic mechanism regulating gene expression in pollen revealed through single-nucleus multiomics

The paper reveals an antagonistic epigenetic mechanism in Arabidopsis pollen where MBD5/6 repress methylated loci while MBD7 promotes demethylation and transcriptional activation.

Nucleosome context regulates chromatin reader preference

The paper investigates how nucleosome context influences chromatin reader binding preferences using biochemical and genomic approaches, revealing that multivalent engagement often has regulatory effects beyond simple additivity.

Ribozyme-based biosensor for imaging gene expression in plants

The paper develops a split ribozyme-based biosensor for in vivo visualization of gene expression in plants, demonstrating its use in transient expression and endogenous gene expression in Arabidopsis thaliana.

The Histone H3-H4 Tetramer is a Copper Reductase Enzyme

The paper shows the histone H3-H4 tetramer acts as a copper reductase enzyme, reducing Cu2+ to Cu1+ and affecting cellular processes dependent on reduced copper.