Wenwei Zhang

Single-Cell Genomics Elucidates Molecular Variations and Regulatory Mechanisms in Circulating Immune Cells

The paper introduces CIMA, a multi-omics atlas of human immune cells, identifying regulatory mechanisms and genetic associations across sex, age, and genetic factors.

Efficiently Constructing Complete Genomes with CycloneSEQ to Fill Gaps in Bacterial Draft Assemblies

The paper evaluates CycloneSEQ long-read sequencing for improving bacterial genome assemblies, showing it can produce complete circular genomes when combined with short-read data.

Revolutionizing large-scale DNA synthesis with microchip-based massive in parallel synthesis system

This paper introduces a microchip-based DNA synthesis system that significantly improves DNA product concentration and simplifies downstream processes for large-scale gene synthesis.

A single-molecule nanopore sequencing platform

This paper introduces an improved nanopore sequencing platform called CycloneSEQ, demonstrating long-duration sequencing and high yield with applications in genomics.

Towards Practical and Robust DNA-based Data Archiving Using "Yin-Yang Codec" System

This paper introduces a DNA-based data archiving system called Yin-Yang Codec, which achieves high recovery rates and physical information density in both in vitro and in vivo storage.

Plasma exosomal miRNA analysis of Alzheimer's disease reveals the dysfunction of a neural network

The paper analyzes plasma exosomal miRNA expression in Alzheimer's disease, MCI, and controls to identify miRNA networks associated with AD and propose novel biomarkers.

stLFRsv: a germline SV analysis pipeline using co-barcoded reads

stLFRsv detects structural variations using co-barcoded long reads, achieving 74.2% precision and 22.3% recall for deletions in HG002/NA24385.

Population Bottlenecks and Intra-host Evolution during Human-to-Human Transmission of SARS-CoV-2Population Bottlenecks and Intra-host Evolution during Human-to-Human Transmission of SARS-CoV-2

This study compares SARS-CoV-2 viral populations in COVID-19 patients to show narrow transmission bottlenecks and stochastic dynamics in intra-host and inter-host evolution.

DNB-Based On-Chip Motif Finding (DocMF): a High-Throughput Method to Profile Different Types of Protein-DNA Interactions

The paper introduces DocMF, a high-throughput method to profile protein-DNA interactions, identifying PAM sequences for CRISPR systems and other DNA-binding proteins.

Comparative performance of the BGI and Illumina sequencing technology for single-cell RNA-sequencing

This study compares the performance of BGI and Illumina sequencing technologies for single-cell RNA-sequencing, finding that BGI outperforms Illumina in cell and gene detection but similar in gRNA detection.

Chromosome-level genome assembly of the African pike, Hepsetus odoe

This study presents a chromosome-level genome assembly of the African pike, Hepsetus odoe, using long-read and Hi-C sequencing technologies, providing a resource for evolutionary and functional studies.

CoolMPS™: Advanced massively parallel sequencing using antibodies specific to each natural nucleobase

The paper introduces a new sequencing chemistry (CoolMPS) that improves signal intensity and eliminates scar interference in massively parallel sequencing.

A portable and cost-effective microfluidic system for massively parallel single-cell transcriptome profiling

The DNBelab C4 portable, negative-pressure microfluidic device performs high-throughput single-cell transcriptome profiling, resolving species-specific cells and tissue heterogeneity in murine lung and cerebral cortex comparably to existing platforms.

Development of Coupling Controlled Polymerizations by Adapter-ligation in Mate-pair Sequencing for Detection of Various Genomic Variants in One Single Assay

This paper introduces a new mate-pair sequencing method called CP-AL that improves DNA-circularization efficiency and reduces contamination, enabling accurate detection of genomic variants.

Efficient long single molecule sequencing for cost effective and accurate sequencing, haplotyping, and de novo assembly

This paper introduces stLFR, a low-cost method for long DNA sequencing that enables accurate variant calling, haplotyping, and de novo assembly.

A new massively parallel nanoball sequencing platform for whole exome research

This study evaluates the BGISEQ-500 platform for whole exome sequencing, finding it comparable to Hiseq4000 in SNP detection and slightly better in indel detection.

Reliable Multiplex Sequencing with Rare Index Mis-Assignment on DNB-Based NGS Platform

The paper evaluates index mis-assignment rates on DNB-based sequencing platforms and finds they are exceptionally low, suggesting reliable multiplex sequencing.

3’ Branch Ligation: A Novel Method to Ligate Non-Complementary DNA to Recessed or Internal 3’OH Ends in DNA or RNA

The paper introduces a novel ligation method called 3BL that allows non-complementary DNA to be ligated to 3' ends of DNA or RNA, improving NGS library construction by reducing adapter dimer issues and increasing yield.

Advanced whole genome sequencing and analysis of fetal genomes from amniotic fluid

This study demonstrates the feasibility of whole genome sequencing of fetal genomes from amniotic fluid, revealing additional genetic and clinical information beyond chromosomal abnormalities.