Ilka Maria IM Axmann

UstiGate: A standardized modular cloning platform for advanced engineering of the fungal chassis Ustilago maydis

The paper introduces UstiGate, a modular cloning platform for engineering Ustilago maydis, enabling multigene integration and standardized genetic parts.

Live-cell imaging enables reporter-free monitoring of the circadian rhythm in individual Synechocystis cells

The paper uses live-cell imaging to monitor circadian rhythms in Synechocystis cells without reporters, finding no circadian gating of cell division but rhythmic changes in refractive index.

Multi-omic data fusion reveals the in vivo enzyme kinetics of Vibrio natriegens at the genome-scale

This study measures in vivo enzyme turnover numbers in Vibrio natriegens using multi-omic data fusion, revealing enzymes are 14-fold faster than E. coli and showing machine learning underestimates these kinetics.

A Spectrum of Possibilities: A Systematic Evaluation of Fluorescent Proteins in Cyanobacteria

This study evaluates fluorescent proteins in cyanobacteria to identify suitable options for research, finding several highly suitable proteins and combinations.

Easy-to-use whole-genome sequencing workflows and standardized practices to uncover hidden genetic variation in Synechocystis PCC 6803 wild-type and knock-out strains

This paper develops workflows for whole-genome sequencing to identify genetic variation in Synechocystis strains and highlights the importance of strain validation in knock-out studies.

Extracellular Polymeric Substances Drive Curvibacter Host Adaptation and Co-Speciation with Hydra

This study shows Curvibacter bacteria co-evolve with Hydra through extracellular polymeric substances, enabling host-specific colonization and symbiosis stability.

Oligonucleotide Library Assisted Sequence Mining Reveals Promoter Sequences With Distinct Temporal Expression Dynamics For Applications In Curvibacter SP. AEP1-3

The paper identifies active promoters in Curvibacter sp. AEP1-3 using an oligonucleotide library and flow cytometry, revealing distinct temporal expression dynamics across growth phases.

Self-sustained rhythmic behavior of Synechocystis PCC 6803 under continuous light conditions in the absence of light-dark entrainment

The paper investigates circadian rhythms in Synechocystis PCC 6803 under continuous light, finding self-sustained 24-hour oscillations without light-dark cycles.

CATHI: An interactive platform for comparative genomics and homolog identification

CATHI provides an intuitive platform for comparative genomics and homolog identification, integrating tools like BLAST and MAFFT to streamline analysis for biologists without programming expertise.

Comparative analysis of inducible promoters in cyanobacteria

The paper evaluates four inducible promoters in Synechocystis sp. PCC 6803 to identify their dynamic range, strength, and toxicity, aiming to improve synthetic biology applications in cyanobacteria.

Quantitative characterization of translational riboregulators using an in vitro transcription-translation system

This paper quantitatively characterizes in silico designed riboregulators using a cell-free TX-TL system to measure their performance and dissociation constants.

6S RNA supports recovery from nitrogen depletion in Synechocystis sp. PCC 6803

This study shows that 6S RNA in Synechocystis sp. PCC 6803 aids recovery from nitrogen depletion by regulating sigma factor switching and maintaining photosynthetic activity.