Anna Delgado-Tejedor

Systematic benchmarking of commercial workflows for isoform-resolved single-nucleus transcriptomics

This study benchmarks four commercial LR snRNA-seq workflows, finding 10x Genomics 3' and 5' to be the most balanced for isoform-resolved single-nucleus transcriptomics.

Single-molecule m6A profiling reveals position-dependent mRNA regulation and non-canonical roles for Ythdf2 in early embryogenesis

The paper uses single-molecule m6A profiling to study mRNA regulation during zebrafish MZT, finding m6A's role in mRNA decay and poly(A) tail dynamics.

SeqTagger, a rapid and accurate tool to demultiplex direct RNA nanopore sequencing datasets

SeqTagger demultiplexes direct RNA nanopore sequencing datasets with high precision and recall, supporting multiple chemistries and library types.

De novo basecalling of m6A modifications at single molecule and single nucleotide resolution

This paper introduces m6ABasecaller, a method for de novo basecalling of m6A modifications at single molecule and single nucleotide resolution using nanopore sequencing.

Enhanced detection of RNA modifications and mappability with high-accuracy nanopore RNA basecalling models

This paper shows that using high-accuracy nanopore RNA basecalling models improves detection of RNA modifications, read mappability, and basecalling accuracy.

Nano3P-seq: transcriptome-wide analysis of gene expression and tail dynamics using end-capture nanopore cDNA sequencing

Nano3P-seq quantifies RNA abundance, tail composition, and tail length dynamics at single-read resolution without PCR or adapters.

Parallel and scalable workflow for the analysis of Oxford Nanopore direct RNA sequencing datasets

The paper introduces a scalable workflow for analyzing Oxford Nanopore direct RNA sequencing data, enabling RNA modification detection and polyA tail length estimation.