This study identifies the origin and renewal mechanisms of gut lacteal smooth muscles from villus myofibroblasts in mice, revealing a NOTCH3-DLL4 signaling role in their development and function.

Developmental Cell · Mar 26, 2024Peer-reviewedDevelopmental Biology

Spatiotemporal transcriptomics reveals pathogenesis of viral myocarditis

Madhav Mantri, Meleana M. Hinchman, David W. McKellar, Michael F. Z. Wang, Shaun T. Cross, John S. L. Parker, Iwijn De Vlaminck

This study uses spatiotemporal transcriptomics to map reovirus-induced myocarditis in neonatal mice, revealing immune responses and cellular interactions in the heart and intestine.

Nature Cardiovascular Research · Oct 10, 2022Peer-reviewedGenomics

Mouse lemur transcriptomic atlas informs primate genes, mutations, physiology, and disease

Camille Ezran, Shixuan Liu, Stephen Chang, Jingsi Ming, Lisbeth A. Guethlein, Michael F. Z. Wang, Roozbeh Dehghannasiri, Julia Eve Olivieri, Hannah K. Frank, Alexander J. Tarashansky, Winston Lian Chye Koh, Qiuyu Jing, Olga Borisovna Botvinnik, Jane Antony, Angela Oliveira Pisco, , Mark A. Krasnow

This paper uses a mouse lemur transcriptomic atlas to identify new genes, splice junctions, and disease-related features, highlighting their relevance to primate biology and human disease.

bioRxiv · Aug 6, 2022 · PreprintGenetics

An organism-wide atlas of hormonal signaling based on the mouse lemur single-cell transcriptome

Shixuan Liu, Camille Ezran, Michael F. Z. Wang, Zhengda Li, Kyle Awayan, Jonathon Z. Long, Iwijn De Vlaminck, Sheng Wang, Jacques Epelbaum, Christin S. Kuo, Jérémy Terrien, Mark A. Krasnow, James E. Ferrell

The paper creates an organism-wide hormone signaling atlas in mouse lemurs, identifying hormone-producing and -target cells and revealing species-specific rewiring and conservation compared to humans and mice.

bioRxiv · Dec 13, 2021 · PreprintGenomics

Uncovering Transcriptional Dark Matter via Gene Annotation Independent Single-Cell RNA Sequencing Analysis

Michael F. Z. Wang, Madhav Mantri, Shao‐Pei Chou, Gaetano J. Scuderi, David W. McKellar, Jonathan Talbot Butcher, Charles G. Danko, Iwijn De Vlaminck

The paper introduces a method to uncover transcriptionally active regions in scRNA-seq data beyond standard gene annotations, improving transcriptome coverage in understudied organisms.

Nature Communications · Apr 12, 2021Peer-reviewedBioinformatics

Spatiotemporal single-cell RNA sequencing of developing hearts reveals interplay between cellular differentiation and morphogenesis

Madhav Mantri, Gaetano J. Scuderi, Roozbeh Abedini‐Nassab, Michael F. Z. Wang, David W. McKellar, Hao Shi, Benjamin Grodner, Jonathan Talbot Butcher, Iwijn De Vlaminck

This study uses spatiotemporal single-cell RNA sequencing to analyze chicken heart development, revealing the interplay between cellular differentiation and morphogenesis.

Nature Communications · Mar 19, 2021Peer-reviewedGenomics

Strength in numbers: Large-scale integration of single-cell transcriptomic data revealsrare, transient muscle progenitor cell states in muscle regeneration

David W. McKellar, Lauren D. Walter, Leo T. Song, Madhav Mantri, Michael F. Z. Wang, Iwijn De Vlaminck, Benjamin D. Cosgrove

The paper integrates large-scale single-cell and spatial transcriptomic data to identify rare, transient muscle progenitor cell states involved in muscle regeneration.

bioRxiv · Dec 1, 2020 · PreprintGenomics

Simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells

Mridusmita Saikia, Philip Burnham, S. Keshavjee, Michael F. Z. Wang, Michael Heyang, Pablo Moral-López, Meleana M. Hinchman, Charles G. Danko, John S. L. Parker, Iwijn De Vlaminck

This paper introduces DART-seq, a method that enables multiplexed amplicon sequencing and transcriptome profiling in single cells, applied to study viral transcripts and B cell immune repertoires.

Nature Methods · Dec 5, 2018Peer-reviewedGenomics