The paper maps the endomembrane system of cerebellar Purkinje neurons using electron microscopy, revealing cell-type-specific and subcellular structural organization of organelles.

bioRxiv · Jul 8, 2025 · PreprintNeuroscience

Development of a Charge-Resistant Embedding Media for High-Performance Serial Block Face Imaging of Cells and Tissues

Thomas J. Deerinck, Steven T. Peltier, Mark H. Ellisman

This paper develops a PEG-doped resin to reduce specimen charging in SBEM, improving imaging quality without sacrificing resolution or physical properties.

bioRxiv · May 28, 2025 · PreprintCell Biology

Do perineuronal nets stabilize the engram of a synaptic circuit?

Varda Lev‐Ram, Sakina P. Lemieux, Thomas J. Deerinck, Eric A. Bushong, Alex J. Perez, Denise R. Pritchard, Brandon H. Toyama, Sung Kyu R. Park, Daniel B. McClatchy, Jeffrey N. Savas, Michael A. Whitney, Stephen Adams, Mark H. Ellisman, John R. Yates, Roger Y. Tsien

The paper investigates whether perineuronal nets (PNNs) stabilize synaptic circuits and engram formation through high-resolution imaging and experimental approaches.

Cells · Sep 29, 2024Peer-reviewedNeuroscience

Genetic requirements for cell division in a genomically minimal cell

James F. Pelletier, Lijie Sun, Kim S. Wise, Nacyra Assad-Garcia, Bogumil Jacek Karas, Thomas J. Deerinck, Mark H. Ellisman, Andreas Mershin, Neil Gershenfeld, Ray-Yuan Chuang, John I. Glass, Elizabeth A. Strychalski

Seven genes, including ftsZ, sepF and five of unknown function, are jointly required to restore near-normal morphology and division in the minimal cell JCVI-syn3.0.

Cell · Mar 29, 2021Peer-reviewedSynthetic Biology✦ In-depth digest