Daniel A. Fletcher

This paper shows that tall adhesion proteins can overcome steric inhibition of phagocytosis by promoting close contact between macrophages and target cells in crowded environments.

This study visualizes actin conformation dynamics in CTLs and shows they precede force generation during immune synapse formation.

This study uses deep learning and physics-based modeling to engineer a CD27 variant that improves the efficacy of CD70-targeting CAR T-cells across multiple cancer models.

This study investigates how osmotic pressure and micropipette aspiration affect liquid-liquid phase transitions in giant unilamellar vesicles, finding both can increase transition temperatures.

This study shows influenza A virus actively transports itself by modifying its environment, using its envelope proteins to create directional cues and bias movement.

The paper describes a new algorithm and field trial for detecting high Loa loa microfilaria levels using the NTDscope device, achieving high sensitivity and specificity.

This paper introduces LUCas, a light-controlled Cas13a system that suppresses background activity and enables precise control of RNA detection, improving assay sensitivity and enabling multiplexed viral co-infection detection.

The paper develops a bead-based Cas13a assay called SurfCas for sensitive and multiplexed RNA detection, showing it matches bulk reaction sensitivity and works in complex biological samples.

This paper develops a theoretical model to simulate phagocytosis of deformable targets and identifies three mechanical regimes based on target stiffness, validated experimentally with GUVs and lymphoma cells.

This study shows that target cell cortical tension determines whether macrophages trogocytose or phagocytose, with low tension favoring trogocytosis and high tension favoring phagocytosis.

SurFlex microscopy quantifies flexibility of surface-tethered biomolecules using fluorescence anisotropy, revealing sequence-dependent DNA flexibility and changes in glycan flexibility on cell surfaces.

This study shows cell surface crowding reduces cell-cell fusion efficiency across multiple systems and identifies it as a tunable biophysical barrier.

This study shows influenza A viruses use a burnt-bridge Brownian ratchet mechanism for directed motion through mucus, operating in an optimal parameter range due to evolutionary adaptation.

CellScape generates editable 2D molecular cartoons from atomic coordinates and combines them into cellular scenes using vector graphics.