Matthew L. Bochman

DNA binding contributes to dominant-negative effects of a catalytically inactive RecQ4-family helicase

The paper investigates how a catalytically inactive RecQ4-family helicase causes dominant-negative effects in DNA repair by showing DNA binding contributes to toxicity.

Declaration of Fermentation: Community-Embedded Wild Yeast Bioprospecting as a Model for Place-Based CURE Design

The paper presents a place-based CURE model where students isolate and deploy wild yeast, confirming its identity and partnering with local breweries to create a historical ale, with sensory validation and design principles outlined.

Genome-wide mapping of helicase-generated ssDNA reveals Hrq1 activity at RNA polymerase III-transcribed genes

The paper maps helicase activity genome-wide using AID-mediated mutational footprints, revealing Hrq1's association with RNA polymerase III-transcribed genes.

Genome-scale characterization of wild yeasts reveals cryptic diversity and population structure across three genera

This study uses genome-scale sequencing to reveal hidden diversity and population structure in wild yeasts, uncovering mixed cultures, a rare species, and a new population while showing that some apparent gene flow signals are artifacts.

FIDDL: depth-matched negative controls distinguish genuine interspecific introgression from competitive-mapping artifact

The paper introduces FIDDL, a tool to distinguish genuine interspecific introgression from artifacts by using depth-matched negative controls and locus-level tests.

A genetically buffered helicase network promotes tolerance of G-quadruplex stabilization in Saccharomyces cerevisiae

This paper investigates G-quadruplex tolerance in yeast by testing helicase mutants, finding that redundancy across multiple helicases, not single families, is required for G4 resolution.

Vibrio campbellii encodes a distinct set of type III secretion system effectors that mediate cytotoxicity in eukaryotic host models

This study identifies four T3SS-secreted effectors in V. campbellii that exhibit cytotoxicity in eukaryotic cells, highlighting conserved T3SS structure but variable effector repertoires across the Harveyi clade.

A screen for synthetic genetic interactions with the Saccharomyces cerevisiae hrq1ΔN allele

This paper identifies synthetic genetic interactions of the hrq1{Delta}N allele in yeast, revealing distinct roles of the Hrq1 NTD in genome integrity pathways.

Dimerization of Cdc13 is essential for dynamic DNA exchange on telomeric DNA

This paper shows that Cdc13 dimerization is required for dynamic DNA exchange on telomeres, using biochemical assays and mutants.

Comprehensive synthetic genetic array analysis of alleles that interact with mutation of the Saccharomyces cerevisiae RecQ helicases Hrq1 and Sgs1

The paper performs synthetic genetic array analysis to investigate genetic interactions of RecQ helicases Hrq1 and Sgs1 in yeast, identifying novel functional connections.

The genetic and physical interactomes of the Saccharomyces cerevisiae Hrq1 helicase

This study defines the Hrq1 interactome in yeast using multi-omics approaches, revealing its role in DNA repair, transcription, and ICL repair, and linking it to human RECQL4 disease mechanisms.

Promoter boundaries for the luxCDABE and betIBA-proXWV operons in Vibrio harveyi defined by the method RAIL: Rapid Arbitrary PCR Insertion Libraries

The paper introduces RAIL, a method for constructing promoter-reporter libraries in Vibrio harveyi to identify promoter boundaries and regulatory sequences.