Fereshteh Dorazehi

A framework for efficient CRISPRi-mediated silencing of retrotransposons in human pluripotent stem cells

This paper describes a protocol for CRISPRi-mediated silencing of retrotransposons in hiPSCs, validated using a multiome approach combining RNA-seq, CUT&RUN, and proteomics.

LINE-1 retrotransposons regulate the exit of human pluripotency and early brain development

This study shows that L1 retrotransposons influence human pluripotency exit and early brain development through their expression and derived transcripts.

MORC2 directs transcription-dependent CpG methylation of human LINE-1 transposons in early neurodevelopment

This study shows MORC2 regulates CpG methylation of active LINE-1 retrotransposons during human neurodevelopment, similar to the piRNA pathway in the germline.

DNA methylation governs the sensitivity of repeats to restriction by the HUSH-MORC2 corepressor

The paper shows that DNA methylation and the HUSH-MORC2 complex independently regulate repeat elements in human neural progenitor cells, with MORC2 binding increasing in demethylated regions.