Roger A. Barker

This paper describes a method to generate dense neuronal microcultures by combining fibroblast reprogramming with matrix remodeling in collagen microgels.

SpiraMed improves brain therapy delivery by using a helical catheter system that enhances coverage and reduces reflux and bleeding risks.

This study identifies BCOR mutations in hiPSCs as a culture-adaptive state causing chromatin, transcriptomic, and proteomic changes, and develops a qPCR assay for detection.

The paper investigates TE transcription in Parkinson's disease brains, finding region- and cell-type-specific TE activation linked to an interferon response in microglia and neurons.

The paper benchmarks dopaminergic stem cell models and cell therapy candidates using a human brain atlas to identify off-target populations and lineage plasticity.

The paper identifies phosphoproteomic changes in Huntington's disease patient-derived neurons, revealing dysregulated post-translational modifications and potential compensatory mechanisms.

TEsingle provides locus-specific transposable element expression analysis in single-cell data, revealing cell-type-specific TE expression in Parkinson's Disease.

This study maps cell-type specific transcriptional changes in the parkinsonian striatum across species, identifying vulnerable and resilient cell types.

This study shows that L1 retrotransposons influence human pluripotency exit and early brain development through their expression and derived transcripts.

OTTR combines live imaging and spatial transcriptomics to link cell behavior to molecular states in human tissues.

This study uses single-cell transcriptomics to compare in vitro and in vivo chondrocyte differentiation, identifies off-target differentiation, and improves in vitro protocols by inhibiting FOXO1.

The paper generates a single-cell and spatial transcriptomic atlas of human fetal meninges and finds that meningiomas likely originate from dura lineage cells.

This study develops a 3D microculture method to generate mature dopamine neurons from patient fibroblasts, which can be transplanted into a Parkinson's disease rat model to form functional neuron grafts.

This study creates a single-cell spatial atlas of the developing human reproductive tract, identifying genes and mechanisms involved in sexual dimorphism and organ development.

This study shows that restoring Fbxo7 expression in dopaminergic neurons of a mouse Parkinson's model rescues tyrosine hydroxylase levels in the striatum and nucleus accumbens.

This study constructs a multiomic atlas of human early skeletal development using single-nuclei transcriptomics, epigenomics, and spatial transcriptomics to uncover regulatory mechanisms and cell states involved in bone and joint formation.

The paper shows that sequencing ambiguity between GBA1 and its pseudogene GBAP1 affects transcriptomic analysis, revealing non-lysosomal functions in the brain and widespread annotation inaccuracies.

This study identifies six novel genetic loci associated with Parkinson's disease mortality and motor progression using genome-wide survival analysis.

This study generates human cerebral organoids with a structured outer subventricular zone by activating the STAT3 pathway with LIF, mimicking in vivo oRG development and enhancing neuronal maturation.

The paper assesses the immunogenicity of RC17 hESC-derived dopaminergic neural progenitor cells, finding they are immunosuppressive and similar to fetal tissue, suggesting reduced need for aggressive immunosuppression post-transplantation.

This paper creates a human embryonic limb cell atlas using single-cell and spatial transcriptomics, identifying novel cell populations and developmental patterns.

This study shows L1 retrotransposons contribute to human brain transcriptome complexity and functional diversification through cell-type specific transcripts and regulatory RNAs.

This study creates a comprehensive cell atlas of the first-trimester human brain using single-cell RNA sequencing and spatial transcriptomics, identifying over 600 distinct cell states and their spatial organization.

This study creates a prenatal human skin cell atlas, revealing immune cells' role in skin development and showing their impact on vascular and hair follicle formation.

The paper constructs a single-cell chromatin accessibility atlas of the human first trimester brain, identifies regulatory elements linked to gene expression, and links disease SNPs to potential pathogenic mechanisms.

The paper shows SARM1 is present in oligodendrocytes but not Schwann cells, and its deletion does not affect myelination in zebrafish or mice.

The paper constructs a human fetal lung cell atlas from 5-22 post conception weeks, identifying new cell states and regulators of epithelial fates.

This study compares three cortical organoid protocols to assess brain regional identity, cell type specificity, and neuronal maturation, finding that directed differentiation methods yield higher regional identity while default methods produce broader cell type diversity.

This study identifies an FBXO7 mutation that disrupts its interaction with PI31, affecting proteasome and mitochondrial function in patient cells.

This study shows GAPDH facilitates EV biogenesis and enables efficient siRNA delivery to the brain, reducing huntingtin gene expression in a mouse model.