The paper uses CRISPRa to activate Tfeb in a rat model of alpha-synuclein aggregation but finds limited protein expression and no additive effects with media treatments.

bioRxiv · Oct 19, 2026 · PreprintMolecular Biology

A systematic screening assay identifies efficient small guide RNAs for CRISPR activation

Elin Arvidsson, Diana Duarte Lobo, Ermelinda Sabarese, Fábio Teixeira Duarte, Rui Jorge Nobre, Cecilia Lundberg, Luís Quintino

The paper presents a screening assay to identify efficient sgRNAs for CRISPR activation and provides a cloning workflow for viral vector integration.

Frontiers in Bioengineering and Biotechnology · Jan 23, 2025Peer-reviewedMolecular Biology

Aβ/APP-induced hyperexcitability and dysregulation of homeostatic synaptic plasticity in models of Alzheimer's disease

Isak Martinsson, Luís Quintino, Megg G. Garcia, Sabine C Konings, Laura Torres-Garcia, Alexander Svanbergsson, Oliver Stange, Rebecca England, Tomas Deierborg, Jiayi Li, Cecilia Lundberg, Gunnar K. Gouras

This study shows that APP and Aβ increase neuronal hyperexcitability and impair homeostatic synaptic and intrinsic plasticity in Alzheimer's disease models.

Frontiers in Aging Neuroscience · Jul 6, 2022Peer-reviewedNeuroscience

Rational design of a compact CRISPR-Cas9 activator for AAV- mediated delivery

Suhani Vora, Jenny Yeuk‐Ki Cheng, Ru Xiao, Nathan J. VanDusen, Luís Quintino, William T. Pu, Luk H. Vandenberghe, Alejandro Chavez, George McDonald Church

The paper designs a compact CRISPR-Cas9 activator suitable for AAV-mediated delivery and validates its transcriptional activation efficacy in multiple cell lines.

bioRxiv · Apr 15, 2018 · PreprintSynthetic Biology