Chemically modified CRISPR-Cas9 enables targeting of individual G-quadruplex and i-motif structures, revealing ligand-dependent transcriptional perturbation.
Sabrina Pia Nuccio, Enrico Cadoni, Roxani Nikoloudaki, Silvia Galli, An-Jie Ler, Claudia Sanchez-Cabanillas, Thomas E. Maher, Ella Fan, Dilek Guneri, Gem Flint, Minghui Zhu, Ling Sum Liu, Christopher R. Fullenkamp, Zoë A. E. Waller, Luca Magnani, , Marco Di Antonio
This paper introduces ATENA, a method using chemically modified CRISPR-Cas9 to target specific G-quadruplexes and i-motifs, showing ligand-dependent transcriptional changes in c-MYC.
Nature Communications · Dec 9, 2025Peer-reviewedCell Biology