Calvin Tiengwe

Chemotactic responses of Trypanosoma brucei procyclic forms to proline and other metabolites

This study shows Trypanosoma brucei procyclic forms respond to metabolites like proline and succinate through chemotaxis, involving cAMP signaling and specific proteins.

A luminal proteome of the endoplasmic reticulum and Golgi apparatus reveals a novel modulator of ER stress tolerance in African trypanosomes

This study maps the luminal proteome of the ER and Golgi in T. brucei using TurboID proximity biotinylation, identifying TbMANF as a novel modulator of ER stress tolerance in African trypanosomes.

An octadecameric O-glucosyltransferase generates diversity in antibody epitopes on variant surface antigens in African trypanosomes

This study identifies ESAG3 as an O-glucosyltransferase that modifies VSG3 to alter antibody epitopes and influence infection outcomes in African trypanosomes.

DishCam: an open-source 3D-printed darkfield imager for microbial colonies and small organisms on Petri dishes

DishCam is an open-source 3D-printed darkfield imager for improved imaging of microbial colonies and small organisms on Petri dishes, offering high contrast and compatibility with incubators.

A high-throughput protein tagging toolkit that retains endogenous UTRs for studying gene regulation in Kinetoplastids.

The paper introduces a CRISPR/Cas9-based protein tagging system for Kinetoplastids that preserves endogenous regulatory elements and demonstrates its utility in studying gene regulation.

Generation of a bloodstream form Trypanosoma brucei double glycosyltransferase null mutant competent in receptor-mediated endocytosis of transferrin

This paper creates double null mutants of TbGT8 and TbGT10 in Trypanosoma brucei, showing reduced pNAL synthesis but normal TfR function and parasite viability.