Takuya Azami

Simplified In Vitro Generation of Human Gastruloids for Modelling Early Development

This paper optimizes a protocol for generating human gastruloids by adjusting culture conditions, improving efficiency and cost-effectiveness for studying early development.

Array-CNCC: precise aggregation and arrayed plating facilitate quantitative phenotyping of human cranial neural crest cells and craniofacial disease modelling

WNT-mediating TCF/LEF transcription factor gene expression in early human pluripotency and cell lineages differs from the rodent paradigm

The paper compares WNT/{beta}-catenin signalling and TCF/LEF gene expression in human and mouse pluripotent stem cells, revealing species-specific differences in early development.

A new resource of clonal pluripotent human stem cell lines exhibiting inter- and intra-embryo consistency and variability

The paper describes a resource of clonal human pluripotent stem cell lines derived from embryos, showing intra- and inter-embryo variation and potential for modeling chromosomal mosaicism.

STAT3 signalling enhances tissue expansion during postimplantation mouse development

This study shows that STAT3 signaling is crucial for tissue expansion during postimplantation mouse development by promoting cell proliferation and differentiation.

A single-cell atlas of pig gastrulation as a resource for comparative embryology

This study creates a single-cell transcriptomic atlas of pig gastrulation, revealing conserved and divergent gene programs and fate dynamics across species.

Requirement for STAT3 and its target, TFCP2L1 in self-renewal of naïve pluripotent stem cells in vivo and in vitro

The paper shows STAT3 and TFCP2L1 are essential for epiblast self-renewal during diapause, but only TFCP2L1 is needed for deriving embryonic stem cells in vitro.

Distinct phospho-variants of STAT3 regulate naïve pluripotency and developmental pace in vivo

This study shows that STAT3 phosphorylation variants regulate naïve pluripotency and developmental timing in mouse embryos, with loss of active STAT3 causing developmental delay and metabolic defects.