Wentao Yang

This study determines the structural basis of DNA N6-adenine methylation by modeling the AMT1 complex and validating its function through mutagenesis.

The paper identifies two distinct methyltransferase complexes that coordinate to maintain DNA N6-methyladenine (6mA) through different mechanisms during replication and transcription.

The paper develops a novel 6mA-calling pipeline for SMRT sequencing data, improving accuracy and efficiency, and applies it to map nucleosome positioning and transcription factor occupancy at single-molecule resolution.

The paper demonstrates that DNA N6-adenine methylation (6mA) is transmitted semi-conservatively in Tetrahymena thermophila, with AMT1-dependent maintenance and AMT1-independent de novo methylation mechanisms.

The paper presents a strategy for telomere-to-telomere assembly of ciliate macronuclear genomes using ultra-high coverage Nanopore data, successfully assembling complete genomes of two ciliate species.

The paper compares nucleosome distribution patterns between two nuclei in Tetrahymena thermophila, finding distinct positioning in the active MAC and delocalized patterns in the silent MIC.