Jing Fan

Multi-omic analysis reveals nitric oxide dependent remodeling in classically activated macrophages and identifies negative regulation mediated by AKR1A1

This study uses multi-omic analysis to show NO* drives controlled remodeling in macrophages, with AKR1A1 mediating negative regulation of metabolic and functional changes during immune response.

Discovery of metabolites produced by reactions between central carbon metabolites and cysteine that mark inflammatory macrophages

The paper identifies sulfur-containing metabolites specific to inflammatory macrophages, showing they are produced from cysteine and central metabolism intermediates and are elevated in human inflammatory lesions.

The pyruvate branch point controls lymphoid cancer cell dissemination

This study identifies the pyruvate branch point as a metabolic checkpoint controlling lymphoid cancer cell migration and dissemination via mROS/HIF-1a signaling.

Acetyl-CoA availability regulates neuronal metabolism, growth, and synaptic activity

This study shows that acetyl-CoA availability, regulated by AT-1, affects neuronal metabolism, growth, and synaptic activity in mice.

Label free metabolic imaging to enhance the efficacy of Chimeric Antigen Receptor T cell therapy

Autofluorescence imaging informs manufacturing conditions that enhance yield and metabolic fitness of CAR T cells for neuroblastoma.

Single cell autofluorescence imaging reveals immediate metabolic shifts of neutrophils with activation across biological systems

This study uses optical metabolic imaging to show neutrophils rapidly shift to a reduced redox state upon activation, with heterogeneous responses across cells and donors.

Mitochondrial metabolism is rapidly re-activated in mature neutrophils to support stimulation-induced response

This study shows that mature neutrophils can rapidly reactivate mitochondrial metabolism in response to stimuli, impacting their function and suggesting therapeutic potential for mitochondrial pyruvate carrier inhibitors.

GATA1-deficient human pluripotent stem cells generate neutrophils with improved antifungal immunity that is mediated by the integrin CD18

This study shows that GATA1-deficient iPSC-derived neutrophils have enhanced antifungal activity against Aspergillus fumigatus, mediated by CD18 integrin.

Multi-parametric thrombus profiling microfluidics detects intensified biomechanical thrombogenesis associated with hypertension and aging

This paper introduces a microfluidics-based thrombus profiling assay that detects intensified biomechanical thrombogenesis in hypertension and aging, revealing a GPIb-integrin mechanosensing axis hyperactivity mechanism.

Fast and Deep Phosphoproteome Analysis with the Orbitrap Astral Mass Spectrometer

The paper introduces a method using the Orbitrap Astral mass spectrometer with DIA to rapidly analyze phosphoproteomes, detecting over 80,000 phosphorylation sites in mice.