Adam Rosenthal

Temporal dynamics of RNA phage infection reveal bacterial recovery despite ongoing viral replication

The paper studies RNA phage infection dynamics in E. coli, showing bacterial population rebound despite viral replication and cell death.

(p)ppGpp-dependent activation of gene expression during nutrient limitation

This study identifies a mechanism in Bacillus subtilis where (p)ppGpp links reduced protein synthesis during nutrient limitation to the activation of specific genes like hpf, facilitating survival and developmental responses.

Simultaneous measurements of translation rate and transcriptome in single bacterial cells by scTR-seq uncovers linked regulation within a specialized active cell population.

The paper measures translation rates and transcriptomes in single bacterial cells to identify a subpopulation with increased protein translation and specific gene overexpression under nutrient limitation.

Recurrent Extinction of Resistance Mutations Leads to Convergent Multidrug Resistance in Sequential Antibiotic Treatment

The paper shows that sequential antibiotic treatment leads to convergent multidrug resistance through extinction of resistance mutations, with single-cell RNA sequencing revealing metabolic and translational state changes.

BacSC: A general workflow for bacterial single-cell RNA sequencing data analysis

BacSC is a computational pipeline for bacterial single-cell RNA sequencing data that automates quality control, dimension reduction, and clustering, improving subpopulation detection across multiple bacterial species.