Squire J. Booker

This paper isolates and characterizes BchE, a cobalamin-dependent radical SAM enzyme, enabling in vitro studies of its role in bacteriochlorophyll biosynthesis.

The paper introduces a synthetic lipid analog to quantify reaction intermediates and products in the Tetraether Synthase reaction, revealing a new GDGT-to-mAG ratio.

This paper investigates the role of amino acids in the RSSY motif of lipoyl synthase, showing that Arg306 and Ser308 are critical for substrate binding and catalytic activity, with specific substitutions affecting product formation and cluster stability.

This paper reports the structural and spectroscopic analysis of a class C radical SAM methylase involved in Nosiheptide biosynthesis, revealing key catalytic residues and radical mechanisms.

The paper investigates how ArsL suppresses 5'-dA* formation and discovers an organometallic species involving ACP and the [Fe4S4] cluster to facilitate carbon-arsenic bond formation.

The paper determines the Cryo-EM structure of Cfr, revealing its mechanism of C8 methylation on 23S rRNA and its role in antibiotic resistance.

This paper reports X-ray crystal structures of a cobalamin-dependent radical S-adenosylmethionine enzyme, revealing how it facilitates iterative methylation of substrates and cobalamin.

This study identifies two critical residues in FDX1 essential for both cuproptosis and lipoylation through deep mutational scanning.