Steven Henikoff

This study shows that Firre RNA has trans-acting effects on the inactive X chromosome, affecting histone modifications, PRC2 complex components, and chromatin structure.

This study shows that H3.1 and H3.3 K27M oncohistones affect chromatin landscapes differently in DMG cells, with H3.1K27M leading to loss of H3K27 trimethylation.

This paper develops scCUT&Tag to profile H3K27me3 in single cells, distinguishing cell types in blood and identifying PcG activity changes in brain tumors before and after treatment.

The paper introduces a modified CUT&Tag method that produces chromatin accessibility maps comparable to ATAC-seq by adjusting tagmentation conditions for H3K4me2 or H3K4me3.

The paper shows RNA stabilizes heterochromatin domains by acting as a structural scaffold, using CUT&RUN.RNase to study RNA's role in chromatin organization.

The paper introduces four enhancements to the CUT&RUN method to improve chromatin profiling accuracy and efficiency.

The paper introduces CUT&Tag, a method for high-resolution epigenomic profiling of small samples and single cells with low background and efficient library preparation.

The paper introduces an automated CUT&RUN platform to profile chromatin modifications in human cell lines and tumor samples, revealing cell-type specific gene regulatory programs.

The paper introduces an improved CUT&RUN protocol that enables high-quality epigenomic profiling with low cell numbers and reduced experimental time.

The paper introduces CUT&RUN, a method for high-resolution mapping of DNA binding sites with lower sequencing depth and background compared to ChIP, and demonstrates its effectiveness in yeast and human nuclei.

This study uses ChEC-seq to identify two classes of transcription factor binding sites in budding yeast based on cleavage kinetics, revealing that DNA sequence and shape influence TF-DNA interactions.