Gabriel E. Zentner

CDK12/13 inhibition disrupts nucleolar morphology and promotes aberrant expression of IGS transcripts

This study shows CDK12/13 inhibition disrupts nucleolar structure and causes aberrant IGS rRNA transcript expression due to impaired processing and degradation.

Renal L-2-hydroxyglutarate dehydrogenase activity promotes hypoxia tolerance and mitochondrial metabolism in Drosophila melanogaster

This study shows that L2hgdh in Drosophila renal tubules prevents L-2HG accumulation, supports mitochondrial metabolism, and enables hypoxia tolerance.

Glycolytic Disruption Triggers Interorgan Signaling to Nonautonomously Restrict Drosophila Larval Growth

The paper shows that disrupting glycolysis in Drosophila larvae leads to systemic cytokine signaling changes, nonautonomously restricting growth.

Generating and testing reagents for CRISPR/Cas9 based homologous recombination and gene drive in Tribolium

The paper attempts to induce Cas9-dependent homologous recombination and gene drive in Tribolium castaneum but finds no evidence of homologous recombination or genetic drive.

Generating and testing the efficacy of transgenic Cas9 in Tribolium castaneum

The paper generates and tests transgenic Cas9 lines in Tribolium castaneum to improve CRISPR editing efficiency.

Connection of core and tail Mediator modules restrains transcription from TFIID-dependent promoters

The paper investigates the effects of separating Mediator modules on transcription, finding that tail/cMed disconnection leads to overactivation of TFIID-dependent genes and underactivation of SAGA-requiring genes.

Flexible analysis of TSS mapping data and detection of TSS shifts with TSRexploreR

The paper introduces TSRexploreR, an R package for analyzing TSS mapping data, including TSS/TSR detection, normalization, and shift detection.

ChEC-seq produces robust and specific maps of transcriptional regulators

The paper reanalyzes ChEC-seq data to show that short-duration experiments produce specific transcription factor binding maps in budding yeast.

Architectural Mediator subunits are differentially essential for global transcription in yeast

The paper shows that Med14 and Med17 subunits of the Mediator complex are essential for transcription in yeast, with Med17 having a more severe impact.

Simple and efficient measurement of transcription initiation and transcript levels with STRIPE-seq

STRIPE-seq provides a simple, rapid, and cost-effective method for TSS profiling and transcript level measurement in yeast and human cells.

A Cyclin A - Myb-MuvB - Aurora B network regulates the choice between mitotic cycles and polyploid endoreplication cycles.

The paper identifies a Cyclin A - Myb-MuvB - Aurora B network that regulates the switch from mitotic to endoreplication cycles in Drosophila, showing its role in both developmental and induced endoreplication.

Mediator is essential for small nuclear and nucleolar RNA transcription in yeast

The paper shows that the Mediator complex is required for sn/snoRNA transcription in yeast, similar to its role in mRNA transcription.

Expanding the genetic toolkit of Tribolium castaneum

This paper introduces new genetic tools for Tribolium castaneum, including GAL4 drivers, fluorescent reporters, and CRISPR-mediated markers, to enhance genetic analysis and toolkit development.

CRISPR/Cas9 gene drives in genetically variable and non-randomly mating wild populations

The paper shows that genetic variation in Cas9 target sites and inbreeding can reduce the efficacy of CRISPR/Cas9 gene drives in wild populations.

ChEC-seq kinetics discriminates transcription factor binding sites by DNA sequence and shape in vivo

This study uses ChEC-seq to identify two classes of transcription factor binding sites in budding yeast based on cleavage kinetics, revealing that DNA sequence and shape influence TF-DNA interactions.