Author

Hardik Makkar

University of PennsylvaniaORCID 0000-0002-8416-4138Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 4 Organism:human· 3 Research Focus:cell type annotation· 2 Research Focus:drug response / drug discovery· 2 Assay:proteomics· 2 Research Focus:cancer & tumor microenvironment· 1 Research Focus:cell signaling & trafficking· 1 Research Focus:chromatin & epigenetic regulation· 1

Frequent Co-Authors

Kyle H. ViningiD· 4 shared Kang I. KoiD· 2 shared Nghi M. TraniD· 2 shared Rebecca G. WellsiD· 2 shared Shuchen ZhangiD· 2 shared Yi CheniD· 2 shared Amanda S. Bluem· 1 shared Chider CheniD· 1 shared

Activity by Year

2025-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 27 mutual co-author links

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4 shared papersBioengineering

Kyle H. ViningORCID

Mutual co-authors in cluster

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4 papers

Recent·Most cited

Nuclear confinement from matrix stiffness drives epigenomic reprogramming of gingival fibroblasts

Hardik Makkar, Kang I. Ko, Rebecca G. Wells, Kyle H. Vining

This study shows that matrix stiffness influences epigenomic changes in gingival fibroblasts, with stiff matrices promoting CpG methylation and reduced inflammation, while soft matrices lead to hypomethylation and increased inflammatory signaling.

bioRxiv · May 31, 2026 · PreprintBioengineering

Mechanical licensing of functional dendritic cell states for enhanced T cell priming

Yi Chen, Amanda S. Bluem, Fatemeh Tavakoli Joorabi, Kexin Zhang, Nghi M. Tran, Shuchen Zhang, Hardik Makkar, Kyle H. Vining

This paper shows that mechanical stress relaxation in dendritic cells (DCs) influences their functional states, enhancing T cell priming through improved antigen presentation and migration.

bioRxiv · May 23, 2026 · PreprintBioengineering

Mineralized Tissue-Targeting Expression System for Local Control of Gene Expression

Jingyi Liu, Qinyuan Chen, Il‐Chul Yoon, Hardik Makkar, Shilan Zhang, Yu‐Chang Chen, Yuantong Li, Jedtanut Thussananutiyakul, Lulu Xue, Shuchen Zhang, Junchao Xu, Yan Luo, Keyu Chen, Michael J. Mitchell, Chider Chen, Kyle H. Vining

This paper develops a gene expression system targeting mineralized tissues using bisphosphonate lipids, demonstrating enhanced RNA delivery and local therapeutic effects in bone regeneration and inflammation.

bioRxiv · Nov 25, 2025 · PreprintBioengineering

Matrix Stiffness Governs Fibroblast-Driven Immune Homeostasis in Gingival Tissues

Hardik Makkar, Nghi Tran, Y. Chen, Kang I. Ko, Rebecca G. Wells, Kyle H. Vining

This study shows that matrix stiffness influences fibroblast-driven immune responses in gingival tissues, with stiff matrices reducing inflammation through non-canonical NFκB and epigenetic pathways.

bioRxiv · Oct 20, 2025 · PreprintBioengineering