Nicholas R. Marzano

Direct single-molecule visualization of Hsp90-mediated relief of a Hsp70-folding block

This study uses single-molecule techniques to show how Hsp90 helps Hsp70 release folding intermediates and remodel client proteins to enable proper refolding.

Single-molecule visualisation of human Hsp70-driven conformational remodelling during stress

This study uses smFRET to visualize how Hsp70 remodels misfolded Fluc under chemical and heat stress, revealing conformational expansion and Hsp110-driven release for productive folding.

Small heat shock proteins HspB1 and HspB5 differentially alter the condensation and aggregation of the TDP-43 low complexity domain

This paper shows that HspB1 and HspB5 differentially regulate TDP-43LCD condensation and aggregation, with HspB1 increasing dynamic exchange and HspB5 delaying pathological transition.

Competing chaperone pathways in α-synuclein disaggregation and aggregation dynamics

This study investigates the interaction between sHsps and Hsp70 chaperones in α-synuclein disaggregation and aggregation, finding no synergy and potential antagonism between the chaperone classes.

Single-molecule observations of human small heat shock proteins in complex with aggregation-prone client proteins

This study uses single-molecule techniques to characterize how human sHsps bind to aggregation-prone client proteins and form complexes.

Single-molecule visualization of sequence-specific RNA binding by a designer PPR protein

This study investigates how designer PPR proteins bind to specific RNA sequences, showing they do not scan longer RNAs and instead rely on diffusion, with implications for their localization in organelles.

Distinct molecular and functional properties of human induced-proprioceptor and low-threshold mechanoreceptor neurons

This study generates human proprioceptor and low-threshold mechanoreceptor neurons from pluripotent stem cells and characterizes their molecular and functional properties.