The paper examines sex- and fiber type-specific proteome changes in human skeletal muscle after resistance training, finding greater adaptations in type II fibers and sex differences in intermediate filament remodeling.

Nature Communications · Sep 2, 2026Peer-reviewedPhysiology

Integrated Single-Fiber Multi-Omics Links an Inflammatory-Associated Myofiber State to Altered Myosin Dynamics in Patients with ICU-acquired weakness

Alexandra Marie Winant, Roger Moreno‐Justicia, Lucia Paolini, Wout J. Claassen, Coen A. C. Ottenheijm, Atul S. Deshmukh, Stefano Cattaneo, Simone Piva, Nicola Latronico, Robert A. E. Seaborne, Julien Ochala

This study identifies an inflammatory myofiber state in ICU-acquired weakness linked to altered myosin dynamics through integrated single-fiber multi-omics analysis.

bioRxiv · Feb 17, 2026 · PreprintCell Biology

Deconvoluting fiber type proportions from human skeletal muscle transcriptomics and proteomics data using FibeRtypeR

Thibaux Van der Stede, Roger Moreno‐Justicia, Freek Van de Casteele, Eline Lievens, Alexia Van de Loock, Jonas Vandecauter, Peter Merseburger, Jimmy Van den Eynden, Delphi Van Haver, An Staes, Simon Devos, Morten Hostrup, Pieter Mestdagh, Jo Vandesompele, Ben Stocks, , Wim Derave

This paper introduces FibeRtypeR, a tool to estimate muscle fiber type proportions from bulk transcriptomics and proteomics data using single-fiber reference profiles.

bioRxiv · Sep 17, 2025 · PreprintPhysiology

Body Fluid Proteomic Landscape of Acute Exercise

Nigel Kurgan, Jeppe Kjærgaard Larsen, Naja Zenius Jespersen, Virginia Díez‐Obrero, Roger Moreno‐Justicia, Sarah Elizabeth Heywood, Cecilie Bergmann Lindqvist, Lisa Ottander, Diana Samodova, Nina Sloth Nielsen, Cody Garett Durrer, Mathias Ried‐Larsen, Simon Rasmussen, Ruth J. F. Loos, Roelof A. J. Smit, , Atul S. Deshmukh

This study maps the proteomic changes in plasma, saliva, and urine after acute exercise, identifying over 3,000 regulated proteins and their associations with disease and drug targets.

bioRxiv · May 28, 2025 · PreprintPhysiology

Integrated single cell functional-proteomic profiling of human skeletal muscle reveals a shift in cellular specificity in nemaline myopathy

Robert A. E. Seaborne, Roger Moreno‐Justicia, Jenni M. Laitila, Christopher T. A. Lewis, Lola Savoure, Edmar Zanoteli, Michael William Lawlor, Heinz Jungbluth, Atul S. Deshmukh, Julien Ochala

This paper introduces SMPFO, a method combining fluorescence assays and proteome profiling to study skeletal muscle, revealing reduced myofiber subtype divergence in nemaline myopathy.

The Journal of Physiology · May 1, 2025Peer-reviewedPhysiology

Dysregulated Skeletal Muscle Myosin Super-relaxation and Energetics in Type II Diabetes

Christopher T. A. Lewis, Roger Moreno‐Justicia, Lola Savoure, Enrique Calvo, Agata Bak, Jenni M. Laitila, Robert A. E. Seaborne, Steen Larsen, Hiroyuki Iwamoto, Marina Cefis, José A. Morais, Gilles Gouspillou, Jorge Alegre‐Cebollada, Thomas James Hawke, Jesús Vázquez, , Julien Ochala

This study investigates altered myosin super-relaxation and energy metabolism in type II diabetes using muscle biopsies and proteomic analyses, finding increased super-relaxation and reduced ATP demand.

Diabetologia · Apr 28, 2025Peer-reviewedCell Biology

Mass-spectrometry based proteomics reveals mitochondrial supercomplexome plasticity

Alba González-Franquesa, Ben Stocks, Sabina Chubanava, Helle B. Hattel, Roger Moreno‐Justicia, Lone Peijs, Jonas T. Treebak, Juleen Rae Zierath, Atul S. Deshmukh

The paper uses mass spectrometry to analyze mitochondrial supercomplexes in mice, revealing dynamic changes in subunit composition and new protein associations during exercise training.

Cell Reports · May 1, 2021Peer-reviewedMolecular Biology