Paulina H. Wanrooij

The Y951N patient mutation inactivates the intramolecular switch in human mitochondrial DNA POLgamma

The study shows the Y951N mutation in POLgamma impairs its ability to switch between DNA synthesis and degradation, causing mtDNA depletion without directly affecting polymerase or exonuclease activity.

Biochemical analysis of the endoribonuclease activity of the human mitochondrial topoisomerase 1

This study investigates the endoribonuclease activity of hTop1mt and finds it less efficient than hTop1, explaining the tolerance of rNMPs in mtDNA.

The specific AMPK activator A-769662 ameliorates pathological phenotypes following mitochondrial DNA depletion

This study shows that the AMPK activator A-769662 improves mitochondrial function and partially restores mtDNA levels in cells with mtDNA depletion.

A small molecule inhibitor Mirin prevents TOP3A-dependent mtDNA breakage and segregation

Mirin prevents mtDNA breakage and immune response in MGME1-deficient cells through MRE11-independent mechanisms involving TOP3A and mtDNA replication termination.

PrgE: an OB-fold protein from plasmid pCF10 with striking differences to prototypical bacterial SSBs

The paper characterizes PrgE, a non-essential SSB from plasmid pCF10, showing it binds both single- and double-stranded DNA with unique quaternary assembly and DNA-binding properties.

The integrity and assay performance of tissue mitochondrial DNA is considerably affected by choice of isolation method

This study compares DNA isolation methods to identify one that produces higher integrity mitochondrial DNA suitable for downstream assays.

The physiological level of rNMPs present in mtDNA does not compromise its stability

This study shows that physiological levels of rNMPs in mtDNA do not compromise its stability across mouse lifespan.