Ryan Yue Muller

This study investigates how PABPC1 binding and 3' UTR sequences influence deadenylation rates, revealing two mechanisms by which UTR sequences modulate deadenylation kinetics.

This paper develops a protein-replacement platform to study PABPC function by degrading endogenous PABPC1/4 and expressing engineered variants, revealing domain-specific roles and functional divergence.

The paper shows that slow translation elongation reduces protein output per mRNA, independent of mRNA stability, and demonstrates a dynamic balance between initiation and elongation rates.

The paper uses CiBER-seq to analyze how genetic perturbations affect ISR dynamics in yeast, finding that translation-related perturbations increase ISR activation while ribosome biogenesis impairments reduce it.

The paper introduces CiBER-seq, a method combining CRISPRi and barcoded expression reporters to measure transcriptional responses in human cells, revealing regulatory networks and cell-type-specific factors in NF-κB signaling.

The paper identifies a fungal mutation that allows survival on plants producing toxic rocaglates by altering eIF4A, a target of these compounds.

The paper develops a genome-scale CRISPRi guide library for yeast, validates its coverage through fitness defects, and improves guide measurement accuracy with barcoding and in vitro transcription.

CiBER-seq quantifies genetic perturbation effects on gene expression in yeast by linking CRISPRi guides to phenotypes via barcoded sequencing.

This study characterizes ten homoserine lactone synthases in E. coli, showing their compatibility with the Lux pathway and highlighting disposal risks of HSLs.