Zuriah A. Meacham

Translation elongation as a rate limiting step of protein production

The paper shows that slow translation elongation reduces protein output per mRNA, independent of mRNA stability, and demonstrates a dynamic balance between initiation and elongation rates.

Viral delivery of compact CRISPR-Cas12f for in vivo gene editing applications

This paper demonstrates efficient in vivo CRISPR gene editing using a compact Cas12f system delivered via AAV, showing safety and targeting multiple cell types.

Surveying the global landscape of post-transcriptional regulators

The paper identifies post-transcriptional regulators in budding yeast by analyzing protein fragments and their effects on mRNA, revealing modular domains and interactions involved in mRNA regulation.

A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeast

The paper develops a genome-scale CRISPRi guide library for yeast, validates its coverage through fitness defects, and improves guide measurement accuracy with barcoding and in vitro transcription.

CiBER-seq dissects genetic networks by quantitative CRISPRi profiling of expression phenotypes

CiBER-seq quantifies genetic perturbation effects on gene expression in yeast by linking CRISPRi guides to phenotypes via barcoded sequencing.

An Accessible Continuous-Culture Turbidostat for Pooled Analysis of Complex Libraries

This paper describes a continuous-culture turbidostat system for analyzing complex genetic libraries in yeast and bacteria, demonstrating its use in growth rate analysis, protein fusion selection, and mutational studies.

Double Stranded DNA Damage and Genome Editing Trigger Ribosome Remodeling and Translational Shutdown

This study shows that DNA double-strand breaks cause ribosome remodeling and translational shutdown, independent of p53 and preceding transcriptional changes.