Kun Leng

This study identifies 772 genes regulating the interferon-responsive microglia state using CRISPRi screening in human iPSC-derived microglia, including novel regulators like CNOT10.

This study shows that old mice experience long-term memory issues after stroke due to increased synapse removal by inflammatory cells, which can be mitigated by activating 7-nAchRs.

This study shows that blocking fibrin-induced microglia activation reduces neuronal hyperactivity and restores the neuroimmune interactome in Alzheimer's disease.

The paper uses CRISPRi to screen ASD risk genes in microglia, identifying ADNP's role in synaptic pruning and endocytosis.

The paper uses snRNA-seq to analyze mosaic chromosome 1q gain in a case of epilepsy with hyaline protoplasmic astrocytopathy, finding cell-type-specific gene expression changes in neurons and astrocytes.

The paper shows that loss of GRN in human brain organoids leads to TDP-43 pathology, driven by astrocytes, and can be rescued by progranulin.

This paper develops compact CRISPRi sgRNA libraries and optimal effectors to improve knockdown consistency and efficiency in mammalian cells.

The paper uses CRISPRi screens and single-cell transcriptomics in human astrocytes to identify regulators of inflammatory reactive states and their relevance in disease.

This study uses a CRISPRi/a platform in iPSC-derived microglia to identify genes regulating microglia states and their therapeutic targets.

The paper performs genome-wide CRISPRi/a screens in human neurons to identify gene functions related to oxidative stress and neurodegenerative diseases, finding that lysosomal failure links to ferroptosis.

The paper identifies molecular signatures of selectively vulnerable neurons in Alzheimer's Disease using single-nucleus RNA sequencing and validates findings with neuropathological methods.