Rocío Castellanos-Rueda

Drug-controlled CAR-T cells through the regulation of cell-cell interactions

This paper develops DROP-CARs, drug-regulated CAR-T cells that control cell-cell interactions using small-molecule switches, showing in vitro and in vivo functionality.

Screening of a pooled library of chimeric antigen receptor T cells based on secretory function

This paper presents a nanovial-based platform for high-throughput functional screening of CAR T cell libraries, identifying CAR constructs with distinct secretion profiles over time.

Modeling CAR Response at the Single-Cell Level Using Conditional Optimal Transport

This paper uses conditional optimal transport to model CAR T cell responses at the single-cell level and predict gene expression for unseen CAR variants.

Dissecting the role of CAR signaling architectures on T cell activation and persistence using pooled screening and single-cell sequencing

speedingCARs: accelerating the engineering of CAR T cells by signaling domain shuffling and single-cell sequencing

speedingCARs uses domain shuffling and single-cell sequencing to engineer CAR T cells, identifying effective variants with diverse transcriptional signatures and T cell phenotypes.