Dino Di Carlo

This study quantifies single-cell contractility in human trabecular meshwork cells under various conditions and finds that contractility is context-dependent, shaped by both extracellular and intracellular factors.

This study shows how PEG hydrogel formulation affects the accumulation of proteins and nanoparticles, with larger molecules requiring more permissive formulations.

This paper enhances homotypic targeting by engineering exosome surfaces with lanthanides, significantly improving cell-exosome interactions and enabling new applications in diagnostics and therapy.

The paper introduces Cell-Cell-Seq to study NK cell activation in defined tumor cell dyads, finding contact-associated activation programs distinct from suspension co-culture.

Cell-Cell-seq maps transcriptional states in interacting cell pairs by profiling defined dyads, revealing emergent gene programs and cross-cell coordination in tumor-T cell interactions.

The paper introduces Lo3DP, a low-cost 3D-printer-based microfluidic platform for lab automation, achieving high accuracy and programmability in droplet manipulation and assays.

This paper develops a nanovial-based platform to identify functional TCRs from unconventional T cells, isolating and validating five novel MAIT TCRs with antigen-specific activity.

This paper presents a nanovial-based platform for high-throughput functional screening of CAR T cell libraries, identifying CAR constructs with distinct secretion profiles over time.

This paper introduces capped nanovials as a scalable platform for single-cell and small colony culture, enabling high-throughput screening of growth and function with improved signal-to-noise ratios and co-culture capabilities.

SEC-seq links single-cell transcriptomes with secretion levels by isolating cells and secretions in Nanovials, enabling analysis of secretory phenotypes and their genetic drivers.

This paper introduces multi-reactive hydrogel nanovials that enable temporal control of secretion capture from antibody-secreting cells, achieving high-purity isolation of antigen-specific hybridomas.

This paper develops amphiphilic microparticles with hydrophilic patches to create tunable picoliter-scale dropicles for improved bioassays.

This paper introduces Nanovials for function-first antibody discovery, enabling high-affinity antibody identification against membrane-bound targets.

This paper introduces flow zoometry, a high-throughput screening method for Drosophila, enabling rapid, automated, and high-resolution analysis of whole animals.