Leonard Schärfen

This study investigates how intron RNA structure affects gene expression using reporter assays and machine learning, finding that base pairing significantly modulates mRNA and protein levels.

This paper describes methods for isolating and analyzing nascent RNA using long-read sequencing in budding and fission yeasts to study RNA processing and transcription dynamics.

The paper develops CoSTseq to study nascent RNA base pairing in yeast, revealing rapid folding dynamics and helicase roles in ribosome biogenesis.

The paper benchmarks eight tools for identifying and quantifying alternative polyadenylation sites from RNA-seq data using real, synthetic, and 3'-end sequencing data.