Bijoyita Roy

This study evaluates the fidelity of uridine analog incorporation during in vitro transcription using different RNA polymerases and introduces a protocol to improve incorporation fidelity without affecting RNA yield.

This paper develops a high-temperature in vitro transcription method using thermostable T7 RNAP to produce low-immunogenicity mRNAs without post-synthesis purification.

The paper investigates how the position of nonsense codons affects readthrough efficiency in yeast, showing that poly(A)-binding protein regulates translation termination.