Jiale Du

This study reveals how MKLN1's discoidin and Kelch domains recognize diverse degrons through multivalent interactions, enabling substrate recruitment by the CTLH-MKLN1 E3 ligase.

The paper identifies LRRC58 as a cysteine-responsive E3 ligase that regulates CDO1 stability via ubiquitylation, revealing a mechanism linking cysteine availability to proteasomal turnover.

This paper develops two nanobody inhibitors of UCH37 to separately target its two Ub-processing activities, enabling their independent study.

The paper uses cryo-EM to visualize the assembly of the human 20S proteasome, revealing how chaperones and propeptides guide subunit incorporation and proteasome activation.